Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 52, Issue 2, Pages 210-217Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2021.1925912
Keywords
Bacillus amyloliquefaciens; high osmotic pressure; tobacco waste; 2; 3-butanediol
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Funding
- Shandong Provincial Science Fund for Distinguished Young Scholars [ZR2020JQ11]
- Young Taishan Scholars [TSQN201909159]
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Strain T4 isolated from tobacco waste is capable of resisting toxic inhibitors and producing 2, 3-butanediol under high glucose concentration, showing potential for bioconversion and biochemicals production from biomass waste.
Microbial biomass and waste materials conversion for biochemicals production has been an alternative for energy conservation and emission reduction. While toxic substances in biomass materials and high osmotic pressure formed in fermentation-based systems block the bioconversion processes of microorganisms. In the present study, strain T4 that isolated from tobacco waste could resist toxic inhibitors such as nicotine and was suitable for generation of 2, 3-butanediol (2, 3-BD) with a high concentration of glucose (up to 20%). 30.06 and 1.54 g/L of 2, 3-BD was generated respectively from 50 g/L of tobacco waste with and without 200 g/L glucose after fermentation for 48 h. Besides, the results of biochemical tests showed that it was gram-positive and able to liquefy gelatin, hydrolyze starch and produce catalases. It could utilize glucose but not lactose as carbohydrates during fermentation. The 16S rRNA sequence and systematic analysis revealed that T4 was identified to be a Bacillus amyloliquefaciens (B. amyloliquefaciens). This work presents a promising model microorganism chassis to use the biomass waste for high value-added biochemicals production.
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