4.8 Article

FVE promotes RNA-directed DNA methylation by facilitating the association of RNA polymerase V with chromatin

Journal

PLANT JOURNAL
Volume 107, Issue 2, Pages 467-479

Publisher

WILEY
DOI: 10.1111/tpj.15302

Keywords

chromatin; DNA methylation; FVE; RRM1; small RNA; SUVH9

Categories

Funding

  1. National Natural Science Foundation of China [32025003]
  2. National Key Research and Development Program of China [2016YFA0500801]
  3. Chinese Ministry of Science and Technology

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The study discovered the interaction between SUVH9 and FVE, showing that FVE promotes the association of Pol V with chromatin and contributes to DNA methylation at a subset of RdDM target loci. The FVE-dependent RdDM target loci are more abundant in gene-rich chromosome arms compared to FVE-independent ones.
Association of RNA polymerase V (Pol V) with chromatin is a critical step for RNA- directed DNA methylation (RdDM) in plants. Although the methylated DNA-binding proteins SUVH2 and SUVH9 and the chromatin remodeler-containing complex DRD1-DMS3-RDM1 are known to be required for the association of Pol V with chromatin, the molecular mechanisms underlying the association of Pol V with different chromatin environments remain largely unknown. Here we found that SUVH9 interacts with FVE, a homolog of the mammalian retinoblastoma-associated protein, which has been previously identified as a shared subunit of the histone deacetylase complex and the polycomb-type histone H3K27 trimethyltransferase complex. We demonstrated that FVE facilitates the association of Pol V with chromatin and thus contributes to DNA methylation at a substantial subset of RdDM target loci. Compared with FVE-independent RdDM target loci, FVE-dependent RdDM target loci are more abundant in gene-rich chromosome arms than in pericentromeric heterochromatin regions. This study contributes to our understanding of how the association of Pol V with chromatin is regulated in different chromatin environments.

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