Journal
PLANT JOURNAL
Volume 106, Issue 5, Pages 1414-1430Publisher
WILEY
DOI: 10.1111/tpj.15245
Keywords
jasmonate; anthocyanin; proanthocyanidin; JAZ; MYB9; telomere‐ binding protein; transcription regulation; apple
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Funding
- National Key Research and Development Program [2018YFD1000200]
- Natural Science Foundation of China [31471854]
- Shandong Province Government [SDAIT-06-03]
- Natural Science Foundation of Shandong Province [ZR2019PC004]
- Ministry of Agriculture of China [CARS-27]
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This study revealed the interaction between the apple telomere-binding protein MdTRB1 and MdMYB9 in regulating the accumulation of anthocyanin and proanthocyanidin. Furthermore, it was found that the JA signaling repressor MdJAZ1 interfered with the interaction between MdTRB1 and MdMYB9, leading to a negative modulation of the biosynthesis of anthocyanin and proanthocyanidin.
Jasmonate (JA) induces the biosynthesis of anthocyanin and proanthocyanidin. MdMYB9 is essential for modulating the accumulation of both anthocyanin and proanthocyanidin in apple, but the molecular mechanism for induction of anthocyanin and proanthocyanidin biosynthesis by JA is unclear. In this study, we discovered an apple telomere-binding protein (MdTRB1) to be the interacting protein of MdMYB9. A series of biological assays showed that MdTRB1 acted as a positive modulator of anthocyanin and proanthocyanidin accumulation, and is dependent on MdMYB9. MdTRB1 interacted with MdMYB9 and enhanced the activation activity of MdMYB9 to its downstream genes. In addition, we found that the JA signaling repressor MdJAZ1 interacted with MdTRB1 and interfered with the interaction between MdTRB1 and MdMYB9, therefore negatively modulating MdTRB1-promoted biosynthesis of anthocyanin and proanthocyanidin. These results show that the JAZ1-TRB1-MYB9 module dynamically modulates JA-mediated accumulation of anthocyanin and proanthocyanidin. Taken together, our data further expand the functional study of TRB1 and provide insights for further studies of the modulation of anthocyanin and proanthocyanidin biosynthesis by JA.
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