Novel Disease Host Resistances in the World Core Collection of Trifolium subterraneum

Glasshouse and field investigations of the phenotypic expressions of resistance of a 97- member World Core Collection of subterranean clover (Trifolium subterraneum) collected from its native Mediterranean habitat and representing approximately 80% of the total genetic diversity within the known 10,000 accessions of the species against the most important damping-off and root rot (Phytophthora clandestina, and Pythium irregulare) and foliar (Kabatiella caulivora, Uromyces trifolii-repentis, and Erysiphe trifoliorum) pathogens were performed. An additional 28 diverse cultivars were also included. Associations of these genotypes among 18 disease parameters and 17 morphological traits, and among these disease parameters and 24 climatic and ecogeographic variables from their collection sites, were examined. Many genotypes showed strong phenotypic expression of novel host disease resistance against one or more pathogens, enabling their potential deployment as disease-resistant parents in subterranean clover breeding programs. These new sources of resistance enable future pyramiding of different resistance genes to improve resistance against these pathogens. Of particular value were genotypes with multiple diseaseresistance across soilborne and/or foliar diseases, because many of these pathogens co-occur. All diseases had some parameters significantly correlated with one or more morphological traits and with one or more sites of origin variables. In particular, there were significant negative correlations between damping-off (i.e., germination) and 8 of the 17 morphological characters. The outcomes of these studies provide crucial information to subterranean clover breeding programs, enabling them to simultaneously select genotypes with multiple resistance to co- occurring soilborne and foliar diseases and desirable traits to offer renewed hope for re-establishing a more productive subterranean clover livestock feedbase despite multiple diseases prevailing widely.

Automated summary

Glasshouse and field investigations were conducted on the phenotypic expressions of resistance of a 97-member World Core Collection of subterranean clover against key pathogens. The study identified genotypes with novel disease resistance, offering potential for improved breeding programs. The findings provide crucial information for selecting genotypes with multiple resistance to co-occurring diseases, offering hope for a more productive subterranean clover feedbase.