The γ-clade HD-Zip I transcription factor MdHB-7 regulates salt tolerance in transgenic apple (Malus domestica)

Aims Salinity plays a crucial role in numerous environmental processes, with extreme concentrations resulting in the serious decline of global agricultural productivity. Plant-specific HD-Zip I transcription factors (TFs), particularly gamma-clade HD-Zip I members, are key for the adaption of plants to various abiotic stresses. Our main purpose is to characterize the function of gamma-clade HD-Zip I members in apple under salt stress. Methods GL-3 and transgenic plants were grown in a hydroponic system or in soil and exposed to the presence or absence of NaCl. Photosynthetic capacity, root damage, Na+ and K+ accumulation, reactive oxygen species (ROS) accumulation, and RNA-seq were investigated. Results MdHB-7 (gamma-clade HD-Zip I TF) expression was induced by salt stress, particularly in the roots. We employed MdHB-7 overexpression and MdHB-7 RNAi transgenic apple plants for the treatments to explore the functions of MdHB-7 under salt stress. Results from the salt treatment and phenotypic evaluation revealed the enhancement of the salt tolerance of apple plants by the MdHB-7 expression. The overexpression of MdHB-7 reduced the salt stress-induced damage to root growth and vitality and maintained ion homeostasis. Moreover, the overexpression of MdHB-7 was observed to promote the detoxification of ROS. Transcriptome and qRT-PCR analysis demonstrate that MdHB-7 overexpression up-regulated the expression of antioxidant enzyme-related genes. MdHB-7-mediated root vitality, antioxidant activity and ion homeostasis were impaired, resulting in MdHB-7 RNAi lines that were more sensitive to salt stress. Conclusions Our results demonstrate the key role of MdHB-7 as a salinity stress tolerance regulator in apple (Malus domestica).

Automated summary

The gamma-clade HD-Zip I transcription factor MdHB-7 plays a crucial role in enhancing salt tolerance in apple plants by maintaining ion homeostasis and promoting ROS detoxification. Overexpression of MdHB-7 up-regulates antioxidant enzyme-related genes and improves root vitality under salt stress, highlighting its potential as a salinity stress tolerance regulator in apple.