Journal
PHYTOPATHOLOGY
Volume 111, Issue 12, Pages 2168-2175Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PHYTO-02-21-0054-SC
Keywords
climate change; disease control; host parasite interactions; oomycetes; pest management
Categories
Funding
- FORMAS [2019-01316, 2018-01420]
- Swedish Research Council [2019-04270]
- NKJ-SNS-Dialogue Biocontrol Network [NKJ-SNS 06]
- Carl Tryggers Stiftelse for Vetenskaplig Forskning [CTS 20:464]
- Crafoord Foundation [20200818]
- Partnerskap Alnarp [1317/Trg, VO/2020, 1353/VO/2021]
- Alnarp Stipendiekommittens
- Scottish Government Rural and Environment Science and Analytical Services Division
- Formas [2018-01420, 2019-01316] Funding Source: Formas
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Spraying potato leaves with double-stranded RNAs targeting essential genes of Phytophthora infestans potentially controls late blight disease. Results demonstrate the potential of spray-induced gene silencing (SIGS) in targeting developmentally important genes in P. infestans to mitigate potato late blight.
Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein beta-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-beta-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.
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