Diterpenoids from Zhumeria majdae roots as potential heat shock protein 90 (HSP90) modulators

Four undescribed and 17 known diterpenoids were isolated from the roots of Zhumeria majdae Rech.f. & Wendelbo. Using 1D and 2D NMR spectroscopy, ECD spectroscopy, and HRESIMS data analysis, the structures of the undescribed compounds were elucidated. The anti-proliferative activity of isolated compounds was evaluated against HeLa and MCF7 cancer cell lines. The binding affinity of all compounds to HSP90, one of the targets for the modern anticancer therapy, was investigated using surface plasmon resonance. The results demonstrated that lanugon Q interacted with the chaperone. To explain its mechanism of action, experimental and computational tests were also conducted.

Automated summary

This study isolated 21 diterpenoids from the roots of Zhumeria majdae, including 4 undescribed compounds. These compounds showed anti-proliferative activity against cancer cells, with one compound binding to HSP90. Experimental and computational tests were conducted to explain the mechanism of action.