Journal
PHOTOSYNTHETICA
Volume 59, Issue 3, Pages 381-398Publisher
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.32615/ps.2021.017
Keywords
chlorophyll fluorescence imaging; hyperspectral imaging; image processing; multicolour fluorescence imaging; stresses; thermal imaging
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Chlorophyll fluorescence is a well-established noninvasive technique for studying photosynthesis and plant physiology, with advancements in technology leading to the development of versatile instruments including imaging systems for real-time analysis of heterogeneous patterns of photosynthetic performance in leaves and algae.
Chlorophyll fluorescence has developed into a well-established noninvasive technique to study photosynthesis and by extension, the physiology of plants and algae. The versatility of the fluorescence analysis has been improved significantly due to advancements in the technology of light sources, detectors, and data handling. This allowed the development of an instrumention that is effective, easy to handle, and affordable. Several of these techniques rely on point measurements. However, the response of plants to environmental stresses is heterogeneous, both spatially and temporally. Beside the nonimaging systems, low- and high-resolution imaging systems have been developed and are in use as real-time, multi-channel fluorometers to investigate heterogeneous patterns of photosynthetic performance of leaves and algae. This review will revise in several paragraphs the current status of chlorophyll fluorescence imaging, in exploring photosynthetic features to evaluate the physiological response of plant organisms in different domains. In the conclusion paragraph, an attempt will be made to answer the question posed in the title.
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