Journal
OPTICS EXPRESS
Volume 29, Issue 9, Pages 13864-13875Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.423740
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Funding
- Canadian Institutes of Health Research [PS 168936]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2018-05788]
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This study demonstrated a UV-PARS and microscopy system capable of virtual H&E 3D imaging of tissues, providing depth-resolved imaging of unstained thick tissues with virtual H&E contrast. The system showed high concordance with traditional staining procedures and confocal fluorescence microscopy.
Hematoxylin and eosin (H&E) staining is the gold standard for most histopathological diagnostics but requires lengthy processing times not suitable for point-of-care diagnosis. Here we demonstrate a 266-nm excitation ultraviolet photoacoustic remote sensing (UV-PARS) and 1310-nm microscopy system capable of virtual H&E 3D imaging of tissues. Virtual hematoxylin staining of nuclei is achieved with UV-PARS, while virtual eosin staining is achieved using the already implemented interrogation laser from UV-PARS for scattering contrast. We demonstrate the capabilities of this dual-contrast system for en-face planar and depth-resolved imaging of human tissue samples exhibiting high concordance with H&E staining procedures and confocal fluorescence microscopy. To our knowledge, this is the first microscopy approach capable of depth-resolved imaging of unstained thick tissues with virtual H&E contrast. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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