Bimodular thrombin aptamers with two types of non-covalent locks

Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Here two types of self-assembling locks were used to create bimodular aptamer constructions. Well-known aptamer to thrombin was chosen as a model object. The assembly of duplex locks was more efficient at low concentrations. The functional activity of aptamer modules was nearly the same as in HD1. However, the affinity of bimodular aptamers with G-quadruplex locks to immobilized thrombin was 5-10 times higher.

Automated summary

Aptamers are structured oligonucleotides that specifically bind their targets, and can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Adding aptamers can create nanostructures that bind specifically to certain targets.