Journal
NUCLEIC ACIDS RESEARCH
Volume 49, Issue 7, Pages 3841-3855Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab184
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Funding
- European Community's Seventh Framework Programme H2020 under iNEXT [653706]
- iNEXT [871037]
- French Infrastructure for Integrated Structural Biology [ANR-10-INSB-05-01]
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BAF protein, encoded by the BANF1 gene, plays crucial roles in chromosome compaction, nuclear envelope reassembly, and repairing of large nuclear ruptures during the cell cycle. Phosphorylation by VRK1 reduces BAF's affinity for dsDNA by 5000-fold, while still maintaining its binding to lamin A/C and emerin.
Barrier-to-autointegration factor (BAF), encoded by the BANF1 gene, is an abundant and ubiquitously expressed metazoan protein that has multiple functions during the cell cycle. Through its ability to cross-bridge two double-stranded DNA (dsDNA), it favours chromosome compaction, participates in post-mitotic nuclear envelope reassembly and is essential for the repair of large nuclear ruptures. BAF forms a ternary complex with the nuclear envelope proteins lamin A/C and emerin, and its interaction with lamin A/C is defective in patients with recessive accelerated aging syndromes. Phosphorylation of BAF by the vaccinia-related kinase 1 (VRK1) is a key regulator of BAF localization and function. Here, we demonstrate that VRK1 successively phosphorylates BAF on Ser4 and Thr3. The crystal structures of BAF before and after phosphorylation are extremely similar. However, in solution, the extensive flexibility of the N-terminal helix alpha 1 and loop alpha 1 alpha 2 in BAF is strongly reduced in di-phosphorylated BAF, due to interactions between the phosphorylated residues and the positively charged C-terminal helix alpha 6. These regions are involved in DNA and lamin A/C binding. Consistently, phosphorylation causes a 5000-fold loss of affinity for dsDNA. However, it does not impair binding to lamin A/C Ig-fold domain and emerin nucleoplasmic region, which leaves open the question of the regulation of these interactions.
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