Journal
NATURE PROTOCOLS
Volume 16, Issue 5, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41596-021-00505-5
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Funding
- Dutch Cancer Foundation (KWF) [10989]
- Horizon 2020 Marie SklodowskaCurie individual fellowship
- Horizon 2020Marie Sklodowska-Curie training program [722729]
- Welfare Foundation for Social & Cultural Sciences, Athens, Greece
- Hellenic Foundation for Research and Innovation (HFRI) [775, 3782]
- National Public Investment Program of the Ministry of Development and Investment of Greece/General Secretariat for Research and Technology [2020S.01300001]
- National Institutes of Health [U01 AG060906, U01 AG060906-02S1, P01 AG017242, R01 AG051729, K99 AG065484]
- NKUA-SARG grant [70/3/8916]
- Marie Curie Actions (MSCA) [722729] Funding Source: Marie Curie Actions (MSCA)
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This study proposes a two-phase algorithmic assessment method to validate the presence of senescent cells and determine the type of senescence by combining different markers and measuring pro-inflammatory activity levels. It aims to improve the characterization of senescence-associated phenotypes and the identification of specific senescent subtypes.
The development of genetic tools allowed for the validation of the pro-aging and pro-disease functions of senescent cells in vivo. These discoveries prompted the development of senotherapies-pharmaceutical interventions aimed at interfering with the detrimental effect of senescent cells-that are now entering the clinical stage. However, unequivocal identification and examination of cellular senescence remains highly difficult because of the lack of universal and specific markers. Here, to overcome the limitation of measuring individual markers, we describe a detailed two-phase algorithmic assessment to quantify various senescence-associated parameters in the same specimen. In the first phase, we combine the measurement of lysosomal and proliferative features with the expression of general senescence-associated genes to validate the presence of senescent cells. In the second phase we measure the levels of pro-inflammatory markers for specification of the type of senescence. The protocol can help graduate-level basic scientists to improve the characterization of senescence-associated phenotypes and the identification of specific senescent subtypes. Moreover, it can serve as an important tool for the clinical validation of the role of senescent cells and the effectiveness of anti-senescence therapies. This protocol describes a two-phase assessment of senescence in cultured cells or clinical specimens by first identifying senescent cells using a combination of markers and then defining the senescent subtype by measuring pro-inflammatory activity.
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