Journal
NATURE NEUROSCIENCE
Volume 24, Issue 5, Pages 746-752Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41593-021-00823-7
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Funding
- National Center for Protein Sciences at Peking University
- Beijing Municipal Science AMP
- Technology Commission [Z181100001318002]
- Beijing Brain Initiative of the Beijing Municipal Science AMP
- Technology Commission [Z181100001518004]
- General Program of National Natural Science Foundation of China [31671118, 31871087, 31925017]
- Science Fund for Creative Research Groups of the National Natural Science Foundation of China [81821092]
- NIH BRAIN Initiative [NS103558]
- Peking-Tsinghua Center for Life Sciences
- State Key Laboratory of Membrane Biology at Peking University School of Life Sciences
- Shanghai Municipal Science and Technology Major Project [2018SHZDZX05]
- Shanghai Pujiang Program [18PJ1410800]
- Peking University Postdoctoral Fellowship
- Alzheimer's Association Postdoctoral Research Fellowship [AARF 19 619387]
- Peking-Tsinghua Center Excellence Postdoctoral Fellowship
- Beijing Nova Program [Z201100006820100]
- Guangdong grant, 'Key Technologies for Treatment of Brain Disorders' [2018B030332001]
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The study developed a genetically encoded GPCR sensor with high specificity, sensitivity, and subcellular resolution for imaging serotonin dynamics in behaving animals. This technology provides new insights into the dynamics and mechanisms of serotonin signaling.
Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB(5-HT)) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB(5-HT) detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution.
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