TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data

We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in alpha beta/gamma delta T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer-even full-length-receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5 ' 10x Genomics platforms. TRUST4 is a computational tool for reconstructing T-cell and B-cell receptor repertoires using bulk and single-cell RNA-seq data.

Automated summary

TRUST4 is an open-source algorithm for reconstructing immune receptor repertoires in alpha beta/gamma delta T cells and B cells, supporting both FASTQ and BAM format, and being faster and more sensitive in assembling full-length receptor repertoires. It can also call repertoire sequences from single-cell RNA-seq data and is compatible with various sequencing platforms.