Journal
NATURE METHODS
Volume 18, Issue 4, Pages 369-+Publisher
NATURE RESEARCH
DOI: 10.1038/s41592-021-01099-2
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Funding
- National Key Research and Development Program of China [2017YFA0505300]
- National Natural Science Foundation of China [32027901, 91954202, 31730054, 31661143041, 31127901, 31700743, 91954201]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB37000000]
- Beijing Municipal Science & Technology Commission [Z191100007219004]
- instrument development project of CAS [GJJSTD20180001, YJKYYQ20180069]
- Youth Innovation Promotion Association of CAS [2013066, 2017135]
- Beijing Nova Program from Beijing Municipal Science & Technology Commission [Z202003]
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The ROSE-Z method achieves super-resolution imaging with a <2 nm axial localization precision using an asymmetric optical scheme and approximately 3,000 photons. This method demonstrates nanoscale three-dimensional and two-color imaging, enhancing the investigation of cellular nanostructures.
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only similar to 3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.
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