4.3 Article

Anti-CD20 therapy corrects a CD8 regulatory T cell deficit in multiple sclerosis

Journal

MULTIPLE SCLEROSIS JOURNAL
Volume 27, Issue 14, Pages 2170-2179

Publisher

SAGE PUBLICATIONS LTD
DOI: 10.1177/13524585211003301

Keywords

Multiple sclerosis; anti-CD20; Breg; CD8 Treg; CD8(+) CD28(-) T cell; disease-modifying therapies

Funding

  1. Roche-Genentech

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Long-term treatment with ocrelizumab significantly increases the levels of CD8(+) CD28(-) regulatory T cells and corrects the deficiencies seen in MS patients before treatment, while causing slight decreases in CD4(+) FOXP3+ regulatory T cells. The homeostatic enrichment of CD8 T regulatory cells provides a mechanism, in addition to B cell depletion, for the therapeutic benefits of anti-CD20 treatment in MS.
Objective: To determine the effect of long-term anti-CD20 B-cell-depleting treatment on regulatory T cell immune subsets that are subnormal in untreated MS patients. Methods: 30 clinically stable MS patients, before and over 38 months of ocrelizumab treatment, were compared to 13 healthy controls, 29 therapy-naive MS, 9 interferon-beta-treated MS, 3 rituximab-treated MS, and 3 rituximab-treated patients with other autoimmune inflammatory diseases. CD8, CD28, CD4, and FOXP3 expression in peripheral blood mononuclear cells was quantitated with flow cytometry. Results: CD8(+) CD28(-) regulatory cells rose from one-third of healthy control levels before ocrelizumab treatment (2.68% vs 7.98%), normalized by 12 months (13.5%), and rose to 2.4-fold above healthy controls after 18 months of ocrelizumab therapy (19.0%). CD4(+) FOXP3(+) regulatory cells were lower in MS than in healthy controls (7.98%) and showed slight long-term decreases with ocrelizumab. CD8(+) CD28(-) and CD4(+) FOXP3(+) regulatory T cell percentages in IFN-beta-treated MS patients were between those of untreated MS and healthy controls. Interpretation: Long-term treatment with ocrelizumab markedly enriches CD8(+) CD28(-) regulatory T cells and corrects the low levels seen in MS before treatment, while slightly decreasing CD4(+) FOXP3+ regulatory T cells. Homeostatic enrichment of regulatory CD8 T cells provides a mechanism, in addition to B cell depletion, for the benefits of anti-CD20 treatment in MS.

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