Journal
MOLECULES
Volume 26, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/molecules26092779
Keywords
Escherichia coli; biotransformation; sodium benzoate; benzoate-CoA ligase; benzophenone synthase; 2; 4; 6-trihydroxybenzophenone; structural annotation
Funding
- Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand
- National Research Council of Thailand, Khon Kaen University, 2018 (NRCT-KKU 2018) [610034]
Ask authors/readers for more resources
A new artificial pathway was established in E. coli to transform sodium benzoate into 2,4,6-trihydroxybenzophenone, paving the way for further syntheses of benzophenone derivatives by adding various catalytic genes for functional groups.
The synthesis of natural products by E. coli is a challenging alternative method of environmentally friendly minimization of hazardous waste. Here, we establish a recombinant E. coli capable of transforming sodium benzoate into 2,4,6-trihydroxybenzophenone (2,4,6-TriHB), the intermediate of benzophenones and xanthones derivatives, based on the coexpression of benzoate-CoA ligase from Rhodopseudomonas palustris (BadA) and benzophenone synthase from Garcinia mangostana (GmBPS). It was found that the engineered E. coli accepted benzoate as the leading substrate for the formation of benzoyl CoA by the function of BadA and subsequently condensed, with the endogenous malonyl CoA by the catalytic function of BPS, into 2,4,6-TriHB. This metabolite was excreted into the culture medium and was detected by the high-resolution LC-ESI-QTOF-MS/MS. The structure was elucidated by in silico tools: Sirius 4.5 combined with CSI FingerID web service. The results suggested the potential of the new artificial pathway in E. coli to successfully catalyze the transformation of sodium benzoate into 2,4,6-TriHB. This system will lead to further syntheses of other benzophenone derivatives via the addition of various genes to catalyze for functional groups.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available