Journal
MOLECULES
Volume 26, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/molecules26082347
Keywords
anticonvulsant; Rosaceae; n-hexane extract
Funding
- Higher Education Commission, Pakistan under NRPU project [6224]
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The study investigated the anti-convulsant effect of the n-hexane extract of Rosa webbiana fruit in an animal model of epilepsy, showing significant impact on convulsive behavior, survival rate of rats, and downregulation of neuro-inflammation, tumor necrosis factor alpha, and nuclear factor kappa B. Gas Chromatography-Mass Spectrometry analysis revealed multiple interesting constituents in the extract, including esters, alkanes, and amines.
Rosa webbiana L. (Rosaceae) is one of the least reported and most understudied members of this family. It is native to the Himalayan regions of Pakistan and Nepal. The anti-convulsant effect of n-hexane extract of fruit of Rosa webbiana was investigated in a pentylenetetrazole (PTZ)-induced animal model of epilepsy. Male Sprague-Dawley rats were divided into six groups (n = 7) including control, PTZ (40 mg/kg), diazepam (4 mg/kg) and n-hexane extract (at 50, 150 and 300 mg/kg). Convulsive behavior was observed and resultant seizures were scored, animals sacrificed and their brains preserved. Chitosan nanoparticles were prepared using the ionic gelation method and characterized by UV-analysis, zeta potential and Fourier transform infrared spectroscopy (FTIR). The effects of all the treatments on the expression of phosphorylated cytokine tumor necrosis factor alpha (p-TNF-alpha) and phosphorylated transcription factor nuclear factor kappa B (p-NF-kappa B) expression in the cortex and hippocampus of the brains of treated rats were studied through enzyme linked immunosorbent assay (ELISA) and morphological differences and surviving neuronal number were recorded through hematoxylene and eosin (H&E) staining. Significant changes in seizures score and survival rate of rats were observed. Downregulation of neuro-inflammation, p-TNF-alpha and p-NF-kappa B was evident. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of this fraction showed multiple constituents of interest, including esters, alkanes and amines.
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