Journal
MOLECULES
Volume 26, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/molecules26102985
Keywords
surfactin; Bacillus subtilis; medium components
Funding
- [008/RID/2018/19]
Ask authors/readers for more resources
The study found that under suitable culture conditions, Bacillus subtilis natto BS19 can efficiently synthesize surfactin using starch as the carbon source and yeast extract as the nitrogen source. Potato peelings were proven to be an effective waste substrate, enhancing the biosynthesis of surfactin.
An effective microbial synthesis of surfactin depends on the composition of the culture medium, the culture conditions and the genetic potential of the producer strain. The aim of this study was to evaluate the suitability of various medium components for the surfactin producing strain and to determine the impact of the culture conditions on the biosynthesis of surfactin isoforms by the newly isolated native strain Bacillus subtilis natto BS19. The efficiency of surfactin biosynthesis was determined by measuring the surface tension of the medium before and after submerged culture (SmF) and by qualitative and quantitative analysis of the obtained compound by high performance liquid chromatography. The highest efficiency of surfactin biosynthesis was achieved using starch as the carbon source and yeast extract as the nitrogen source at pH 7.0 and 37 degrees C. Potato peelings were selected as an effective waste substrate. It was shown that the increase in the percentage of peel extract in the culture medium enhanced the biosynthesis of surfactin (mg/L) (2-30.9%; 4-46.0% and 6-58.2%), while reducing surface tension of the medium by about 50%. The obtained results constitute a promising basis for further research on biosynthesis of surfactin using potato peelings as a cheap alternative to synthetic medium components.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available