Journal
MOLECULAR PLANT PATHOLOGY
Volume 22, Issue 6, Pages 627-643Publisher
WILEY
DOI: 10.1111/mpp.13049
Keywords
antiviral RNA silencing; Argonaute protein; Cymbidium mosaic virus; Odontoglossum ringspot virus; Phalaenopsis aphrodite subsp; formosana; virus‐ induced gene silencing (VIGS)
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Funding
- Advanced Plant Biotechnology Center from The Featured Areas Research Center Program within the Ministry of Education (MOE)
- Innovative Translational Agricultural Research Program [2018PRE022, AS-108-ITAR-L022, AS-109-ITAR-L022]
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This study analyzed the role of AGOs in orchids in defending against major viruses, finding that PaAGO5a and PaAGO5b play central roles in antiviral defense mechanisms. A virus-induced gene silencing vector based on Foxtail mosaic virus was developed to confirm the importance of PaAGO5s in defenses against CymMV and ORSV.
The orchid industry faces severe threats from diseases caused by viruses. Argonaute proteins (AGOs) have been shown to be the major components in the antiviral defence systems through RNA silencing in many model plants. However, the roles of AGOs in orchids against viral infections have not been analysed comprehensively. In this study, Phalaenopsis aphrodite subsp. formosana was chosen as the representative to analyse the AGOs (PaAGOs) involved in the defence against two major viruses of orchids, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). A total of 11 PaAGOs were identified from the expression profile analyses of these PaAGOs in P. aphrodite subsp. formosana singly or doubly infected with CymMV and/or ORSV. PaAGO5b was found to be the only one highly induced. Results from overexpression of individual PaAGO5 family genes revealed that PaAGO5a and PaAGO5b play central roles in the antiviral defence mechanisms of P. aphrodite subsp. formosana. Furthermore, a virus-induced gene silencing vector based on Foxtail mosaic virus was developed to corroborate the function of PaAGO5s. The results confirmed their importance in the defences against CymMV and ORSV. Our findings may provide useful information for the breeding of traits for resistance or tolerance to CymMV or ORSV infections in Phalaenopsis orchids.
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