Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis

Article Medical Laboratory Technology

Rapid and Extraction-Free Detection of SARS-CoV-2 from Saliva by Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification

Matthew A. Lalli et al.

Summary: The study developed a rapid colorimetric assay for SARS-CoV-2 detection using saliva samples without RNA extraction, achieving high accuracy and sensitivity.

CLINICAL CHEMISTRY (2021)

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Article Microbiology

Evaluating 10 Commercially Available SARS-CoV-2 Rapid Serological Tests by Use of the STARD (Standards for Reporting of Diagnostic Accuracy Studies) Method

Laurent Dortet et al.

Summary: This study evaluated and compared 10 commercially available SARS-CoV-2 rapid serological tests using the STARD methodology, finding that only four tests achieved >= 98% specificity and the sensitivities varied by the day of sample collection after the onset of symptoms. Only three of the tests evaluated met French health authorities' thresholds for SARS-CoV-2 serological tests.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Virology

Development of a point-of-care test to detect SARS-CoV-2 from saliva which combines a simple RNA extraction method with colorimetric reverse transcription loop-mediated isothermal amplification detection

Wataru Yamazaki et al.

Summary: The study has successfully developed a simple and rapid method for extracting RNA from saliva and conducting COVID-19 testing, which can be used in field settings to potentially reduce the risk of infection.

JOURNAL OF CLINICAL VIROLOGY (2021)

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Article Virology

Clinical performance of the Panbio assay for the detection of SARS-CoV-2 IgM and IgG in COVID-19 patients

Helene Haguet et al.

Summary: The study evaluated the clinical performance of the Panbio COVID-19 IgG/IgM rapid test, showing high sensitivity for IgG, particularly suitable for testing more than 14 days after symptom onset. However, the sensitivity for IgM was lower, while both IgG and IgM exhibited excellent specificity in the assay.

JOURNAL OF MEDICAL VIROLOGY (2021)

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Article Biochemical Research Methods

Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

Emma L. A. Howson et al.

Summary: A simplified sample preparation method has been optimized for rapid and direct detection of SARS-CoV-2 RNA in saliva using RT-LAMP. This method allows for non-invasive collection of samples without the need for skilled staff, overcoming the bottleneck of extraction robots and reagents, and enabling public health intervention during the COVID-19 pandemic.

JOURNAL OF VIROLOGICAL METHODS (2021)

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Article Multidisciplinary Sciences

Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction

Shan Wei et al.

Summary: The study presents a rapid diagnostic method based on RT-LAMP that can directly detect SARS-CoV-2 in clinical settings in 30 minutes. This method is highly sensitive and robust, making it suitable for worldwide implementation, especially in resource-poor settings.

SCIENTIFIC REPORTS (2021)

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Article Infectious Diseases