Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 41, Issue 8, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1128/MCB.00065-21
Keywords
CEBPA-AS1; miR-24-3p; BOK; I/R; OGD/R; CEBPA-AS1
Categories
Funding
- National Natural Science Foundation of China [81901343]
- Suzhou Science and Technology Project [SYS2020067]
- Nature Science Research Project of Anhui province [1908085QH356]
- Funding of Peak Training Program for Scientific Research of Yijishan Hospital [GF2019G01]
Ask authors/readers for more resources
The downregulation of CEBPA-AS1 protects against OGD/R-induced cell injury by targeting the miR-24-3p/BOK axis, reducing oxidative stress and apoptosis.
Cerebral ischemia/reperfusion (I/R) can lead to serious brain function impairments. Long noncoding RNA (lncRNA) CCAAT enhancer binding protein a antisense RNA 1 (CEBPA-AS1) was shown to be upregulated in human ischemic stroke. This study investigated the function and mechanism of CEBPA-AS1 in I/R. An oxygen-glucose deprivation/reperfusion (OGD/R) model was used to induce I/R injury in SH-SY5Y cells in vitro. RTqPCR examined the expression of CEBPA-AS1, microRNA 24-3p (miR-24-3p), and Bcl-2related ovarian killer (Bok). The cell viability, apoptosis, oxidative stress in OGD/R-treated cells were detected using CCK-8, flow cytometry, Western blotting, and enzyme-linked immunosorbent assays. The relationship among genes was tested by RNA pulldown and luciferase reporter assays. We found that OGD/R upregulated CEBPA-AS1 expression in SHSY5Y cells. Functionally, CEBPA-AS1 depletion ameliorated OGD/R-induced apoptosis and oxidative stress in SH-SY5Y cells by reducing reactive oxygen species production and superoxide dismutase and glutathione. Mechanistic investigations indicated that CEBPAAS1 acts as a sponge for miR-24-3p, and miR-24-3p binds to BOK. Moreover, miR-24-3p upregulation or BOK downregulation antagonized the protective role of CEBPA-AS1 depletion in SH-SY5Y cells exposed to OGD/R. Overall, downregulation of CEBPA-AS1 exerts protective functions against OGD/R-induced injury by targeting the miR-24-3p/BOK axis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available