Journal
MICROCHEMICAL JOURNAL
Volume 164, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2021.106001
Keywords
Cholesterol; Metal-organic framework; Graphene quantum dots; Nanocomposite; Peroxidase-like activity; Hydrogen peroxide
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Funding
- research council of Azarbaijan Shahid Madani University [ASMU/99372-20]
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A nanocomposite of graphene quantum dots/UiO-66 metal-organic framework was synthesized through a solvothermal method, exhibiting enhanced photocatalytic activity in peroxidase mimetic-based fluorescence analysis of H2O2. By developing a fluorescent biosensor, a linear relationship between fluorescence intensity and cholesterol concentration was utilized for indirect determination of cholesterol, showing promising results for cholesterol quantification in human serum samples.
In this study, graphene quantum dots/zirconium-based metal?organic framework (GQDs/UiO-66) nanocomposite was synthesized via a solvothermal procedure in one pot. The photocatalytic activity of the prepared GQDs/UiO-66 nanocomposite in peroxidase-like reactions was investigated using colorimetric and spectrofluorometric techniques. The photocatalytic activity of the nanocomposite in peroxidase mimetic-based fluorescence analysis of H2O2 based on H2O2-terephthalic acid reaction was considerably enhanced in comparison with its components. Owing to the fact that H2O2 could be produced as a result of enzymatic oxidation of cholesterol, and there is a linear relationship between the fluorescence intensity and cholesterol concentration, a fluorescent biosensor was developed for indirect determination of the cholesterol. In the optimal condition, the calibration plot was linear in the concentration range between 0.04 and 1.60 ?mol L-1, with a detection limit of 0.01 ?mol L-1. The method validation was checked by analyzing the NIST SRM 1952a-2, as a certified reference material, and the evolved technique was successfully used for cholesterol quantification in human serum samples.
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