The Himalayan Onion (Allium wallichii Kunth) Harbors Unique Spatially Organized Bacterial Communities

Plant-associated microorganisms are known to contribute with various beneficial functions to the health and productivity of their hosts, yet the microbiome of most plants remains unexplored. This especially applies to wild relatives of cultivated plants, which might harbor beneficial microorganisms that were lost during intensive breeding. We studied bacterial communities of the Himalayan onion (Allium wallichii Kunth), a wild relative of onion native to mountains in East Asia. The bacterial community structure was assessed in different plant microhabitats (rhizosphere, endosphere, anthosphere) by sequencing of 16S rRNA gene fragment amplicons. Targeted bioinformatic analyses were implemented in order to identify unique features in each habitat and to map the overall community in the first representative of the Amaryllidaceae plant family. The highest bacterial diversity was found for bulk soil (Shannon index, H ' 9.3) at the high-altitude sampling location. It was followed by the plant rhizosphere (H ' 8.9) while communities colonizing flowers (H ' 6.1) and the endosphere (H ' 6.5 and 5.6) where less diverse. Interestingly, we observed a non-significant rhizosphere effect. Another specificity of the microbiome was its high evenness in taxonomic distribution, which was so far not observed in plant microbiomes. Pseudomonas was identified among additional 10 bacterial genera as a plant-specific signature. The first insights into the microbiome of a plant in the widespread Allium genus will facilitate upcoming comparisons with its domesticated relatives while additionally providing a detailed microbiome mapping of the plant's microhabitats to facilitate bioresource mining.

Automated summary

The bacterial communities in different microhabitats of Himalayan onion showed varying levels of diversity, with the rhizosphere having the highest diversity. A unique feature of the microbiome was its high evenness in taxonomic distribution, and plant-specific signature bacteria like Pseudomonas were identified. The study provides insights into the microbiome of the Allium genus and can facilitate comparisons with domesticated relatives for bioresource mining.