Direct cueing by pulsed-waterjet treated titanium on fibroblast, osteoblastic and human stem cells for prospective use in dental implants

Pulsed-waterjet (PWJ) technology holds potential for the biologically effective surface treatment of dental titanium implants. In addition to the proliferative response of in vitro models for gingiva (NIH/3T3s) and bone (Saos-2) cells, our study investigates the osteogenic potential of PWJ-treated titanium surfaces by employing human mesenchymal stem cells (hMSCs). The greatest proliferation of fibroblast NIH/3T3 and osteoblast-like Saos-2 cells was elicited on T-1.76 and T-4.83 treatment conditions, respectively. Morphological traits and the nuclear localization of an osteogenic reporter (OSX) indicate preferential osteogenic differentiation on the T2A3 condition. This work demonstrates the potential of PWJ technology for surface modification, supporting its technological translation as a cost-effective, environmentally friendly, and contaminant-free method to incorporate novel bioactive properties into titanium implants. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

Pulsed-waterjet (PWJ) technology shows potential for biologically effective surface treatment of dental titanium implants. The study demonstrates that different treatment conditions can promote cell proliferation and osteogenic differentiation on titanium surfaces, indicating the potential for surface modification and incorporation of novel bioactive properties into implants.