Journal
MARINE DRUGS
Volume 19, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/md19040200
Keywords
red alga; Pyropia pseudolinearis; Uppurui Nori; ACE inhibitory peptides; docking simulation
Categories
Funding
- Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry [27004B]
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The hydrolysates of red algal proteins are good candidates for ACE inhibition. 42 ACE inhibitory peptides were identified from Pyropia pseudolinearis, among which LRM showed high inhibitory activity and was highly conserved in red algae.
More than 7000 red algae species have been classified. Although most of them are underused, they are a protein-rich marine resource. The hydrolysates of red algal proteins are good candidates for the inhibition of the angiotensin-I-converting enzyme (ACE). The ACE is one of the key factors for cardiovascular disease, and the inhibition of ACE activity is related to the prevention of high blood pressure. To better understand the relationship between the hydrolysates of red algal proteins and the inhibition of ACE activity, we attempted to identify novel ACE inhibitory peptides from Pyropia pseudolinearis. We prepared water soluble proteins (WSP) containing phycoerythrin, phycocyanin, allophycocyanin, and ribulose 1,5-bisphosphate carboxylase/oxygenase. In vitro analysis showed that the thermolysin hydrolysate of the WSP had high ACE inhibitory activity compared to that of WSP. We then identified 42 peptides in the hydrolysate by high-performance liquid chromatography and mass spectrometry. Among 42 peptides, 23 peptides were found in chloroplast proteins. We then synthesized the uncharacterized peptides ARY, YLR, and LRM and measured the ACE inhibitory activity. LRM showed a low IC50 value (0.15 mu mol) compared to ARY and YLR (1.3 and 5.8 mu mol). In silico analysis revealed that the LRM sequence was conserved in cpcA from Bangiales and Florideophyceae, indicating that the novel ACE inhibitory peptide LRM was highly conserved in red algae.
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