Macromolecular Photocatalyst for Synthesis and Purification of Protein-Polymer Conjugates

We describe the application of a novel eosin Y (EY)-derived polymer photocatalyst for the synthesis of polymeric bioconjugates. The photocatalyst, a copolymer of eosin Y acrylate and N-isopropylacrylamide, can induce light-mediated reversible-deactivation radical polymerization in biologically benign conditions. Heating the reaction mixture to 37 degrees C causes precipitation of the photocatalyst in a hydrophilic-to-hydrophobic transition, allowing for simple purification of the polymer-protein conjugates via filtration without compromising enzyme activity. We discuss the optimization of the polymerization conditions for imparting control over molecular weight and reaction kinetics and demonstrate the recyclability of the recovered photocatalyst. Overall, this strategy will advance bioconjugate manufacturing through facilitated purification and improved sustainability.

Automated summary

A novel eosin Y-derived polymer photocatalyst is utilized for synthesizing polymeric bioconjugates in this study. The photocatalyst can induce light-mediated reversible-deactivation radical polymerization under biologically benign conditions, allowing for simple purification of polymer-protein conjugates through precipitation. The study also demonstrates the optimization of polymerization conditions for controlling molecular weight and reaction kinetics, along with the recyclability of the recovered photocatalyst, advancing bioconjugate manufacturing.