Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 142, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2021.111044
Keywords
gamma-aminobutyric acid; Glutamic acid; Degree of hydrolysis; Enzymatic hydrolysis; Fermentation
Categories
Funding
- Project 911 - Vietnam International Education Department (VIED), Ministry of Education and Training, Vietnam
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The production of fermented foods enriched with gamma-aminobutyric acid (GABA) is a current trend in the food industry. The use of enzyme hydrolysis and fermentation to increase GABA content in soy milk was studied, with promising results under optimized conditions.
The production of fermented foods enriched with gamma-aminobutyric acid (GABA) is a current trend in the food industry. In this study, the release of free glutamic acid (Glu) and its conversion to GABA in soymilk were conducted through enzymatic hydrolysis using Alcalase (Al) and Flavourzyme (Fl), following by the fermentation using Lb. plantarum LMG6907 (at 30 degrees C for 96 h). Results showed that a degree of hydrolysis of 90% was achieved when soymilk was treated with a mixture of Al:Fl in a ratio 1:1 (final enzyme concentration of 2 mL/100 mL). Both the free Glu and the GABA content were significantly increased in soymilk hydrolysate, which is a product obtained after enzymatic hydrolysis of soymilk. After 72 h of fermentation, the GABA content in soymilk hydrolysate reached 859 mg/L while the remaining Glu was 2467 mg/L. Fermentation of soymilk hydrolysate at the optimal conditions for glutamate decarboxylase activity (initial pH 4.7, 50 degrees C), the enzyme catalyzing the conversion of Glu to GABA, also did not show a higher GABA production.
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