Journal
JOURNAL OF SEPARATION SCIENCE
Volume 44, Issue 12, Pages 2427-2437Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100028
Keywords
authentication; chemical markers; paeony root; selected ion monitoring; traditional Chinese medicine
Categories
Funding
- National Key R&D Program of China [2019YFC1711000, 2019YFC1711400, 2018YFC1707900]
- National Natural Science Foundation of China [82003938]
- National Traditional Chinese Medicine Leading Talents Support Program [2018]
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The strategy integrating in-depth profiling, chemical marker selection, and selected detection was proven effective in authenticating paeony root in prescriptions, demonstrating practical application value for improving the quality of prescriptions.
Authentication of Chinese medicine materials in prescriptions is extremely difficult due to the complicated chemical matrix. A strategy integrating in-depth profiling, chemical marker selection, and selected detection was established and exemplarily applied to authenticate paeony root in ShaoYao-GanCao decoction. First, an ultra-performance liquid chromatography/linear trap quadrupole-Orbitrap method was developed to probe the chemical compositions of the decoction. Second, 20 batches of decoctions prepared from white paeony root and red paeony root were compared by a metabolomics method, and multistep chemometrics analysis distinguished the chemical markers. Third, an ultra-performance liquid chromatography/QDa-selected ion monitoring method was developed to authenticate the paeony root in decoctions. As a result, 161 compounds were characterized, including 84 triterpene saponins, 42 flavonoids, and 10 monoterpenes. Four chemical markers and paeoniflorin were successfully screened out as chemical markers for white paeony root. The selected ion monitoring method easily differentiated authentic decoction (prepared from white paeony root) from fraud decoction (prepared from red paeony root) by monitoring the above five chemical markers. In conclusion, the strategy was proved effective in authentication of paeony root in ShaoYao-GanCao decoction, and it can also be applied to authenticate other Chinese medicine materials in prescriptions, which will greatly avail the quality enhancement of prescriptions.
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