Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 46, Issue 7, Pages 1592-1599Publisher
WILEY
DOI: 10.1002/eji.201546262
Keywords
Chemotaxis; Efferocytosis; Macrophage activation; Peritonitis; Resolution of inflammation
Categories
Funding
- NIH [HL060903]
- American Heart Association
Ask authors/readers for more resources
Efferocytosis has been suggested to promote macrophage resolution programs that are dependent on motility and emigration, however, few studies have addressed directed migration in resolving macrophages. In this report, we hypothesized that efferocytosis would induce differential chemokine receptor expression. Polarized macrophage populations, including macrophages actively engaged in efferocytosis, were characterized by PCR array and traditional transwell motility assays. We identified specific up-regulation of chemokine receptor CXCR4 on both mouse and human macrophages and characterized in vivo expression of CXCR4 in a resolving model of murine peritonitis. Using adoptive transfer and AMD3100 blocking, we confirmed a role for CXCR4 in macrophage egress to draining lymphatics. Collectively these data provide an important mechanistic link between efferocytosis and macrophage emigration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available