Propofol suppresses osteosarcoma cell function by regulating FOXO1/TUSC7

Objectives Accumulated evidence demonstrates that propofol has antitumour roles in various cancers. However, the role of propofol in osteosarcoma is still unclear.Therefore, we aim to determine the role of propofol on osteosarcoma and further explore its potential mechanism. Methods Cell proliferation, migration and invasion of osteosarcoma were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, wound healing and transwell assay, respectively. The interaction between FoxO1 and TUSC7 was determined using luciferase reporter assay and chromatin immunoprecipitation. Results Propofol treatment significantly decreased cell proliferation, migration and invasion in U2OS cells. Propofol promoted TUSC7 expression by enhancing transcriptional factor FOXO1 that leads to inactivation of AKT/GSK3 beta signalling resulting in the suppression of cell proliferation, migration and invasion. Conclusions Propofol suppresses cell proliferation, migration and invasion of osteosarcoma cells through FOXO1/TUSC7 axis by regulating AKT/GSK3 beta signalling.

Automated summary

In this study, propofol was found to suppress cell proliferation, migration, and invasion of osteosarcoma cells through the FOXO1/TUSC7 axis by regulating the AKT/GSK3 beta signaling pathway.