Journal
EUROPEAN JOURNAL OF HAEMATOLOGY
Volume 97, Issue 3, Pages 245-252Publisher
WILEY
DOI: 10.1111/ejh.12713
Keywords
multipotent mesenchymal stromal cells; acute myeloid leukemia; acute lymphoblastic leukemia; chronic myeloid leukemia; cobblestone area forming cells
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Funding
- Russian Foundation for Basic Research [12-04-00457a, 15-04-02514]
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BackgroundThe development of leukemia impairs normal hematopoiesis and marrow stromal microenvironment. The aim of the investigation was to study the ability of multipotent mesenchymal stromal cells (MSCs) derived from the bone marrow of patients with leukemia to maintain normal hematopoietic progenitor cells. MethodsMSCs were obtained from the bone marrow of 14 patients with acute lymphoblastic (ALL), 25 with myeloid (AML), and 15 with chronic myeloid (CML) leukemia. As a control, MSCs from 22 healthy donors were used. The incidence of cobblestone area forming cells (CAFC 7-8 d) in the bone marrow of healthy donor cultivated on the supportive layer of patients MSCs was measured. ResultsThe ability of MSCs from AML and ALL patients at the moment of diagnosis to maintain normal CAFC was significantly decreased when compared to donors. After chemotherapy, the restoration of ALL patients' MSCs functions was slower than that of AML. CML MSCs maintained CAFC better than donors' at the moment of diagnosis and this ability increased with treatment. ConclusionsThe ability of patients' MSCs to maintain normal hematopoietic progenitor cells was shown to change in comparison with MSCs from healthy donors and depended on nosology. During treatment, the functional capacity of patients' MSCs had been partially restored.
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