Journal
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
Volume 49, Issue 3, Pages -Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0300060521997679
Keywords
Chitosan nanoparticle; bone morphogenetic protein-2; basic fibroblast growth factor; adipose tissue-derived stem cell; osteogenesis; osteocalcin; bone sialoprotein
Funding
- Zhejiang Provincial Medical and Healthy Science Foundation of China [2019ZD028]
- National Key Research Program of China [2016YFC1101004]
- National Natural Science Foundation of China [81671918]
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Co-transfection of BMP2 and FGF2 via chitosan nanoparticles significantly up-regulated the mRNA and protein levels of BMP-2 and FGF2, and increased OCN and BSP gene expression, indicating enhanced osteogenesis in human ADSCs in vitro.
Objective To investigate if co-transfection of human bone morphogenetic protein 2 (BMP-2, BMP2) and human fibroblast growth factor 2 (FGF2, FGF2) via chitosan nanoparticles promotes osteogenesis in human adipose tissue-derived stem cells (ADSCs) in vitro. Materials and Methods Recombinant BMP2 and/or FGF2 expression vectors were constructed and packaged into chitosan nanoparticles. The chitosan nanoparticles were characterized by atomic force microscopy. Gene and protein expression levels of BMP-2 and FGF2 in ADSCs in vitro were evaluated by real-time polymerase chain reaction (PCR), western blot, and enzyme-linked immunosorbent assay. Osteocalcin (OCN) and bone sialoprotein (BSP) gene expression were also evaluated by real-time PCR to assess osteogenesis. Results The prepared chitosan nanoparticles were spherical with a relatively homogenous size distribution. The BMP2 and FGF2 vectors were successfully transfected into ADSCs. BMP-2 and FGF2 mRNA and protein levels were significantly up-regulated in the co-transfection group compared with the control group. OCN and BSP mRNA levels were also significantly increased in the co-transfection group compared with cells transfected with BMP2 or FGF2 alone, suggesting that co-transfection significantly enhanced osteogenesis. Conclusions Co-transfection of human ADSCs with BMP2/FGF2 via chitosan nanoparticles efficiently promotes the osteogenic properties of ADSCs in vitro.
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