Journal
JOURNAL OF IMMUNOLOGY
Volume 206, Issue 11, Pages 2614-2622Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.2100036
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Funding
- Deutsche Forschungsgemeinschaft through Fokus-Forderung COVID-19
- German Federal Ministry of Health
- European Union Horizon 2020 Framework Programme for Innovation and Research (Fight-nCoV)
- Ministry for Science, Research and Arts of Baden-Wurttemberg, Germany
- European Commission (HORIZON2020 Project SUPPORT-E) [101015756]
- BMWi-Federal Ministry for Economic Affairs and Energy (COMBI-COV-2)
- Deutsche Forschungsgemeinschaft [CRC1279]
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The study revealed that SARS-CoV-2 neutralization titers remain stable or even increase for at least seven months after diagnosis, showing a positive correlation with age, symptom number, and specific antigen presence. Platform validation indicated the wild-type virus and surrogate neutralization tests had the highest assay performances, but the selection of cutoff titers significantly affected the evaluation of neutralization potency.
The current SARS-CoV-2 pandemic has triggered the development of various SARS-CoV-2 neutralization tests. A wild-type virus (using African green monkey VeroE6 cells), a pseudovirus (using human Caco-2 cells), and a surrogate neutralization test platform were applied to characterize the SARS-CoV-2 neutralization potential of a cohort of 111 convalescent plasma donors over a period of seven months after diagnosis. This allowed an in-depth validation and assay performance analysis of these platforms. More importantly, we found that SARS-CoV-2 neutralization titers were stable or even increased within the observation period, which contradicts earlier studies reporting a rapid waning of Ab titers after three to four months. Moreover, we observed a positive correlation of neutralization titers with increasing age, number of symptoms reported, and the presence of the Rhesus Ag RhD. Validation of the platforms revealed that highest assay performances were obtained with the wild-type virus and the surrogate neutralization platforms. However, our data also suggested that selection of cutoff titers had a strong impact on the evaluation of neutralization potency. When taking strong neutralization potency, as demonstrated by the wild-type virus platform as the gold standard, up to 55% of plasma products had low neutralization titers. However, a significant portion of these products were overrated in their potency when using the surrogate assay with the recommended cutoff titer. In summary, our study demonstrates that SARS-CoV-2 neutralization titers are stable for at least seven months after diagnosis and offers a testing strategy for rapid selection of high-titer convalescent plasma products in a biosafety level 1 environment.
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