Augmentation of chloramphenicol degradation by Geobacter-based biocatalysis and electric field

Electroactive microorganisms and electrochemical technologies have been separately used for environmental remediation such as antibiotics removal, yet the efficiency of coupling these two methods for chlorinated antibiotics removal is poorly known. Here we tested the synergy of Geobacter sulfurreducens PCA, an electroactive bacteria, and an electrical field, on chloramphenicol removal. Removal is increased two-fold by increasing the temperature from 30?C to 37?C. The cyclic voltammograms and chronoamperometry tests demonstrated that G. sulfurreducens PCA catalyzed chloramphenicol chemical reduction with electrode as excusive electron donor. A critical voltage, -0.6 to -0.5 V vs. Ag/AgCl, was discovered for chloramphenicol degradation with an increase of removal rate about 2.62-folds, from 31.06% to 81.41%. Combined removal with both G. sulfurreducens PCA and an electrical field increased the apparent rate constant and reached 82.77% removal at -0.5 V. Specially, the combined removal at -0.5 V even presented more robust removal efficiency compared to -0.6 V (78.64%) without G. sulfurreducens PCA. Mass spectrometry of degradation products indicates the reduction of nitro into amine groups, and dechlorination into less toxic compounds. Overall, combined biocatalysis and an electrical field is a promising method to remove antibiotics from polluted environments.

Automated summary

The synergy of Geobacter sulfurreducens PCA and an electrical field significantly increased the efficiency of chloramphenicol removal, especially at 37°C. By catalyzing the chemical reduction of chloramphenicol, the removal rate was increased to 81.41%, leading to the formation of less toxic compounds.