Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 407, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124599
Keywords
Cd; HRW; BcIRT1; BcZIP2; Ion absorption
Categories
Funding
- China Agriculture Research System [CARS-23-A03]
- National Key Research and Development Program of China [2016YFD0800700-3]
Ask authors/readers for more resources
The study shows that hydrogen-rich water (HRW) plays a positive role in reducing cadmium uptake by the roots of pak choi seedlings, possibly by inhibiting Cd2+ influx and affecting the expression of Cd transporter proteins.
The results of Cd (cadmium) concentration, Cd2+ fluorescent staining, NMT (non-invasive micro-test technology) analysis of Cd absorption revealed the remarkably positive role of HRW in reducing Cd uptake by root of pak choi seedlings. BcIRT1 (iron-regulated transporter 1) and BcZIP2 (zinc-regulated transporter protein 2) are the main Cd transporters in pak choi, but their roles in the process of HRW-reduced Cd uptake is still far from being answered. In this study, we specifically verified the function of IRT1 and ZIP2 in HRW-reduced Cd absorption in pak choi and Arabidopsis thaliana. Heterologous and homologous expression in Arabidopsis thaliana displayed that Cd concentrations in wild-type (Col-0) and transgenic A. thaliana of IRT1 and ZIP2 were significantly reduced by HRW, except for irt1- and zip2-mutant. NMT detection showed that HRW not only decreased Cd2+ influx in root of WT and transgenic lines, but also enhanced the competition between Zn and Cd. Taken together, the HRW-induced reduction of Cd accumulation in plants may be result from depressing the expression of BcIRT1 and BcZIP2 and affecting the preference of BcIRT1 and BcZIP2 in ion uptake.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available