Journal
EUROPEAN JOURNAL OF CONTRACEPTION AND REPRODUCTIVE HEALTH CARE
Volume 21, Issue 6, Pages 436-442Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13625187.2016.1229765
Keywords
Abortion; microbial ecology; mucosal immunology; prostaglandins
Funding
- National Center for Advancing Translational Sciences Gynuity Health Projects LLC [10.13039/ 100006108 [UL1 TR000445], 7001]
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Objectives: The aim of the study was to assess the extent to which misoprostol alters mucosal or systemic immune responses following either buccal or vaginal administration. Methods: This was a prospective, crossover pilot study of 15 healthy, reproductive-age women. Women first received 800 mu g misoprostol either via buccal or vaginal administration and were crossed over 1 month later to receive the drug via the other route. Cervicovaginal lavage samples, cervical Cytobrush samples, cervicovaginal swabs, urine and blood were obtained immediately prior to drug administration and the following day. Parameters assessed included urine and cervicovaginal misoprostol levels, whole blood cytokine responses (by ELISA) to immune stimulation with lipopolysaccharide, peripheral blood and cervical lymphocyte phenotyping by flow cytometry, cervicovaginal antimicrobial peptide measurement by ELISA and vaginal microbial ecology assessment by 16S rRNA sequencing. Results: Neither buccal nor vaginal misoprostol significantly altered local or systemic immune and microbiological parameters. Conclusion: In this pilot study, we did not observe significant alteration of mucosal or systemic immunology or vaginal microbial ecology 1 day after drug administration following either the buccal or vaginal route.
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