Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 407, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124389
Keywords
Flocculation; SERS labels; Fingerprint peak; Identification; Sterilization mechanism
Categories
Funding
- National Natural Science Foundation of China [21677020, 51608078]
- Key Project of Chongqing Science and Technology Commision, China [cstc 2018jscx-mszd0615]
- Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201900841]
- Analytical and Testing Center of Chongqing University
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The study introduced a novel water treatment method that combines specific compounds to achieve the flocculation, sterilization, and identification of bacteria, greatly improving the efficiency of water treatment.
Bacteria, especially antibiotic-resistant bacteria, in water threaten public health in countries. Simultaneous flocculation, sterilization and identification of bacteria are great challenge in water treatment. Herein we presented a three-in-one compound through combining a novel Bacitracin-based flocculant (B-g-PAMDAC) and surface enhanced Raman scattering (SERS) labels, the modified Au@AgNPs using graphene oxide (GO) and 4-mercaptophenylboronic acid (4-MPBA). B-g-PAMDAC with bactericidal groups and microblock structure was synthesized via copolymerization and self-assembly. Its functional groups and microblock structure contributed to the excellent performance in flocculation of bacteria. 4-MPBA as bacterial capture bound to the bacterial cell membrane and contributed to recognition of bacteria in flocculation. Bacteria aggregating around Au@AgNPs resulted in abundant hot spots and strong Raman signals. SERS labels obviously improved the sensitivity, accuracy and stability of bacteria identification even at low bacterial concentration of 1 x 10(3) CFU mL(-1). They presented distinct fingerprints of bacteria, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus and Enterococcus faecalis, in Raman mappings. Bacitracin improved sterilization efficiency of B-g-PAMDAC in four bacteria treatment in terms of sterilization rate and time. beta-galactosidase and respiratory activity of bacteria revealed sterilization mechanism of B-g-PAMDAC that changed permeability of cell membrane before it reduced the respiration activity of bacteria and ruptured cell wall.
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