4.7 Article

ZmPPR26, a DYW-type pentatricopeptide repeat protein, is required for C-to-U RNA editing at atpA-1148 in maize chloroplasts

Journal

JOURNAL OF EXPERIMENTAL BOTANY
Volume 72, Issue 13, Pages 4809-4821

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/jxb/erab185

Keywords

ATP synthase; chloroplast; maize; pentatricopeptide repeat proteins; RNA editing; ZmPPR26

Categories

Funding

  1. National Natural Science Foundation of China [31630053]
  2. National Key Research and Development Program of China [2016YFD0101003]

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ZmPPR26 is essential for the C-to-U RNA editing and chloroplast biogenesis in maize. Loss of function of ZmPPR26 abolishes editing efficiency, leading to a decrease in chloroplast ATP synthase abundance and accumulation of photosynthetic complexes, ultimately resulting in the albino seedling-lethal phenotype.
Pentatricopeptide repeat (PPR) proteins are involved in the C-to-U RNA editing of organellar transcripts. The maize genome contains over 600 PPR proteins and few have been found to function in the C-to-U RNA editing in chloroplasts. Here, we report the function of ZmPPR26 in the C-to-U RNA editing and chloroplast biogenesis in maize. ZmPPR26 encodes a DYW-type PPR protein targeted to chloroplasts. The zmppr26 mutant exhibits albino seedlinglethal phenotype. Loss of function of ZmPPR26 abolishes the editing at atpA-1148 site, and decreases the editing at ndhF-62, rp120-308, rp12-2, rpoC2-2774, petB-668, rps8-182, and ndhA-50 sites. Overexpression of ZmPPR26 in zmppr26 restores the editing efficiency and rescues the albino seedling-lethal phenotype. Abolished editing at atpA-1148 causes a Leu to Ser change at AtpA-383 that leads to a reduction in the abundance of chloroplast ATP synthase in zmppr26. The accumulation of photosynthetic complexes are also markedly reduced in zmppr26, providing an explanation for the albino seedling-lethal phenotype. These results indicate that ZmPPR26 is required for the editing at atpA-1148 and is important for editing at the other seven sites in maize chloroplasts. The editing at atpA-1148 is critical for AtpA function, assembly of ATP synthase complex, and chloroplast biogenesis in maize.

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