Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1642, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2021.462035
Keywords
Cannabinoids; Tandem MS spectroscopy; Cerumen; Forensic analyzis
Funding
- University of Cyprus
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A validated UPLC-MS/MS method was developed for the detection of THC and CBN in cerumen samples, with quantification possible in 75% of cases.
In this study, cerumen, a non-conventional biological secretion, was examined as an alternative matrix for forensic analyzis. A fully validated analytical UPLC-MS/MS method was developed for the detection and quantification of the most prevalent psychoactive illicit drug globe wide, Delta(9)-tethrahydrocannabinol, commonly known as THC, and four major cannabinoids found in cannabis Sativa. The method was validated, and standard external calibration curves were established with correlation coefficients > 0.99. A validated experimental procedure, along with a direct extraction of cannabinoids with acidified acetonitrile resulted in a short total analyzis time and a good extraction efficiency for all the analytes under study. LOD and LOQ values were determined to be 0.01-0.08 pg/mg and 0.04-0.23 pg/mg, respectively. To prove applicability of the proposed assay, volunteers were selected, and cerumen samples were examined for cannabis. The analyzis by use of UPLC-MS/MS indicated that all samples were positive, reporting recent cannabis abuse. Surprisingly, both THC and Cannabinol (CBN) were detected, and quantification was possible in 75% of the cases. (C) 2021 Elsevier B.V. All rights reserved.
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