Journal
JOURNAL OF CELL SCIENCE
Volume 134, Issue 12, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.251264
Keywords
Lamina; Macromolecular assembly; Trypanosomatid; Nuclear organization; Heterochromatin
Categories
Funding
- Medical Research Council [MR/N010558/1]
- Wellcome Trust Investigator award [204697/Z/16/Z]
- Czech Ministry of Education, Youth and Science (MEYS) [OPVVV/0000759]
- Czech BioImaging grant [LM2015062]
- Wellcome Trust [204697/Z/16/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
The nuclear lamina plays crucial roles in maintaining nuclear structure and controlling gene expression. In trypanosomes, the N- and C-termini of NUP-1 act as interaction hubs and impact other components of the lamina and nuclear envelope.
The nuclear lamina supports many functions, including maintaining nuclear structure and gene expression control, and correct spatiotemporal assembly is vital to meet these activities. Recently, multiple lamina systems have been described that, despite independent evolutionary origins, share analogous functions. In trypanosomatids the two known lamina proteins, NUP-1 and NUP-2, have molecular masses of 450 and 170 kDa, respectively, which demands a distinct architecture from the -60 kDa lamin-based system of metazoa and other lineages. To uncover organizational principles for the trypanosome lamina we generated NUP-1 deletion mutants to identify domains and their arrangements responsible for oligomerization. We found that both the N- and C-termini act as interaction hubs, and that perturbation of these interactions impacts additional components of the lamina and nuclear envelope. Furthermore, the assembly of NUP-I terminal domains suggests intrinsic organizational capacity. Remarkably, there is little impact on silencing of telomeric variant surface glycoprotein genes. We suggest that both terminal domains of NUP-1 have roles in assembling the trypanosome lamina and propose a novel architecture based on a hub-and-spoke configuration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available