Journal
BRAIN RESEARCH
Volume 1603, Issue -, Pages 150-157Publisher
ELSEVIER
DOI: 10.1016/j.brainres.2015.01.009
Keywords
Traumatic brain injury; miR-21; Gene therapy; Blood-brain barrier; Secondary brain damage
Categories
Funding
- National Natural Science Foundation of China [81330029, 81471252, 81271361]
- Tianjin Municipal Science and Technology Commission natural fund project [13JCYBJC23700]
- National Clinical Key Subject Construction Project of NHFPC Fund
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Our recent studies have identified increased expression of miR-21 in brain following traumatic brain injury (TBI), which alleviated brain edema that related to the blood-brain barrier (BBB) leakage. To analyze the potential effect of miR-21 on secondary BBB damage after TBI, we employed the fluid percussion injury rat model and manipulated the expression level of miR-21 in brain. We found that miR-21 level in brain microvascular endothelial cells (BMVECs) in lesioned cerebral cortex can be upregulated or downregulated by intracerebroventricular infusion of miR-21 agomir or antagomir. Upregulated miR-21 level conferred a better neurological outcome of TBI, and alleviated TBI-induced secondary BBB damage and loss of tight junction proteins. To explore the molecular mechanism underlying this protective effect, we detected the impact of miR-21 on the expression of Angiopoietin-1(Ang-1) and Tie-2, which can promote the expression of tight junction proteins and amplify BBB stabilization. We found that miR-21 exerts the protective effect on BBB by activating the Ang-1/Tie-2 axis in BMVECs. Thus, miR-21 could be a potential therapeutic target for interventions of secondary BBB damage after TBI. (C) 2015 Elsevier B.V. All rights reserved.
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