Combined multi-band decoupling in biomolecular NMR spectroscopy

Multi-resonance NMR experiments are powerful analytical and structural tools. Their conceptualization assumes that RF fields may be combined independently to manipulate spin interactions. However, practical implementation can compromise performance. One limitation is the generation of combination bands when two or more RF fields are applied simultaneously within the NMR probe. The combination bands can lead to significant interference with the detection circuitry. A facile approach to combined multi-band decoupling can resolve these problems and increase sensitivity two-fold (or more), by time sharing the application of the individual frequencies rather than time sharing decoupling and data acquisition.

Automated summary

Multi-resonance NMR experiments can manipulate spin interactions by independently combining RF fields, but the practical implementation may face interference from combination bands. A facile approach of time sharing the application of individual frequencies can resolve this issue and increase sensitivity.