Journal
JOURNAL OF APPLIED GENETICS
Volume 62, Issue 3, Pages 507-509Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13353-021-00627-2
Keywords
Elephant; Sexing; ZFX; ZFY; KASP probe
Funding
- Economic Commmunity of Central African States (CEEAC) under the ECOFAC V Programme (Fragile ecosystems of Central Africa) - European Union [FED/2013/332-349]
- French Development Agency under the France-Gabon debt conversion [AFD CGA 1188.01.H]
- Department for Environment, Food and RuralAffaires (DEFRA), UK
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A novel real-time PCR assay has been developed for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY. This assay is a simple, inexpensive, and reliable tool suitable for non-invasive DNA samples, and can be incorporated into larger SNP panels for individual identification and population genetic studies.
We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool that is suitable for non-invasive DNA samples and can be incorporated into larger SNP panels for individual identification and population genetic studies.
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