Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 69, Issue 16, Pages 4708-4719Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c00432
Keywords
emulsion interfacial composition; lipid digestion; in vitro; kinetic modeling; gastric lipase; pancreatic lipase
Funding
- Foundation Flanders (FWO) [1S03318N]
- Research Foundation Flanders (FWO) [1222420N]
- Internal Funds KU Leuven
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This research evaluated the impact of emulsion interfacial composition on in vitro small intestinal lipolysis kinetics, finding that the size of emulsion particles affects the kinetics of lipolysis in the small intestinal phase. Multiresponse modeling was used to elucidate the mechanism of lipolysis, including enzymatic and chemical reactions.
This research evaluated the impact of the emulsion interfacial composition on in vitro small intestinal lipolysis kinetics with the inclusion of rabbit gastric lipase resulting in a gastric prelipolysis step. O/w emulsions contained 5% triolein (w/w) and 1% (w/w) of the following emulsifiers: sodium taurodeoxycholate, citrus pectin, soy protein isolate, soy lecithin, and tween 80. Emulsions were subjected to static in vitro digestion and diverse lipolysis species quantified via a HPLC-charged aerosol detector. Single-response modeling indicated that the kinetics of lipolysis in the small intestinal phase were impacted by the emulsion particle size at the beginning of this phase. Multiresponse modeling permitted the elucidation of the lipolysis mechanism under in vitro conditions. The final reaction scheme included enzymatic and chemical conversions. The modeling strategies used in this research allowed to gain more insights into the kinetics and mechanism of in vitro lipid digestion.
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