Cellvibrio polysaccharolyticus sp. nov., a cellulolytic bacterium isolated from agricultural soil

A novel Gram-reaction-negative bacterial strain, designated Ka43(T), was isolated from agricultural soil and characterised using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, the strain shows highest similarity (97.1%) to Cellvibrio diazotrophicus E50(T). Cells of strain Ka43(T) are aerobic, motile, short rods. The major fatty acids are summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH), C-18:1 omega 7c and C-16:0. The only isoprenoid quinone is Q-8. The polar lipid profile includes phosphatidylethanolamine, phosphatidylglycerol, four phospholipids, two lipids and an aminolipid. The assembled genome of strain Ka43(T) has a total length of 4.2 Mb and the DNA G+C content is 51.6 mol%. Based on phenotypic data, including chemotaxonomic characteristics and analysis of the 16S rRNA gene sequences, it was concluded that strain Ka43(T) represents a novel species in the genus Cellvibrio, for which the name Cellvibrio polysaccharolyticus sp. nov. is proposed. The type strain of the species is strain Ka43(T) (=LMG 31577(T)=NCAIM B.02637(T)).

Automated summary

The novel gram-negative bacterial strain Ka43(T) was isolated from agricultural soil and classified as a new species in the genus Cellvibrio, named Cellvibrio polysaccharolyticus sp. nov., based on its unique phenotypic and genotypic characteristics.