Comamonas suwonensis sp. nov., isolated from stream water in the Republic of Korea

A novel bacterial strain, EJ-4(T), isolated from stream water collected at Seo-ho in Suwon, Republic of Korea, was characterized based on a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EJ-4(T) belonged to the genus Comamonas. The isolate is Gram-stain-negative, non-motile, aerobic, rod-shaped and forms pale yellow colonies on trypticase soy agar. The optimal growth of this strain was observed aerobically at 30 degrees C, pH 7 and 0.5% NaCl. The major fatty acids were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c; 39.7 %) and C-16:0 (32.0%). The G+C content of strain EJ-4(T) was 58.4mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain EJ-4(T) and Comamoas testosteroni were 91.8 and 31.2%, respectively. The major polar lipids detected in the isolate were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant isoprenoid quinone was ubiquinone-8. Based on the results of polyphasic taxonomic analysis of strain EJ-4(T), we describe a novel species of the genus Comamonas, for which the name Comamonas suwonensis sp. nov. has been proposed, with EJ-4(T) (=KCTC 82074(T)=JCM 34179(T)=KEMB 1602-279(T)) as the type strain.

Automated summary

Strain EJ-4(T) isolated from stream water in Suwon, Korea, belongs to the genus Comamonas. It is Gram-stain-negative, aerobic, rod-shaped, and grows optimally at 30 degrees C, pH 7, and 0.5% NaCl. The novel species Comamonas suwonensis sp. nov. has been proposed based on polyphasic taxonomic analysis.