Pseudomonas carbonaria sp. nov., isolated from charcoal

A beige-pigmented, oxidase-positive bacterial isolate, Wesi-4(T), isolated from charcoal in 2012, was examined in detail by applying a polyphasic taxonomic approach. Cells of the isolates were rod shaped and Gram- stain negative. Examination of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strains of Pseudomonas matsuisoli and Pseudomonas noso-comialis (both 97.3 %). Phylogenetic analyses on the basis of the 16S rRNA gene sequences indicated a separate position of Wesi-4(T), which was confirmed by multilocus sequence analyses (MLSA) based on the three loci gyrB, rpoB and rpoD and a core genome- based phylogenetic tree. Genome sequence based comparison of Wesi-4(T) and the type strains of P. matsuisoli and P. nosocomialis yielded average nucleotide identity values <95 % and in silico DNA -DNA hybridization values <70 %, respectively. The polyamine pattern con-tains the major amines putrescine, cadaverine and spermidine. The quinone system contains predominantly ubiquinone Q-9 and in the polar lipid profile diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine are the major lipids. The fatty acid contains predominantly C16:0, summed feature 3 (C-16:1 omega 7c and/or C(16:1 omega)6c) and summed feature 8 (C-18:omega 7c and/or C-18:1 omega 6c). In addi-tion, physiological and biochemical tests revealed a clear phenotypic difference from P. matsuisoli. These cumulative data indicate that the isolate represents a novel species of the genus Pseudomonas for which the name Pseudomonas carbonaria sp. nov. is proposed with Wesi-4(T) (=DSM 110367T=CIP 111764T=CCM 9017T) as the type strain.

Automated summary

The bacterial isolate Wesi-4(T) from charcoal in 2012 was identified as a novel species in the genus Pseudomonas, named Pseudomonas carbonaria sp. nov., based on detailed polyphasic taxonomic analysis.