Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/ijms22095045
Keywords
ex vivo; wound model; antimicrobial; biofilm; burn; antibiotics; wound healing
Funding
- Alfred Osterlunds Foundation
- Edvard Welanders Stiftelse
- Crafoord Foundation
- Royal Physiographic Society
- Swedish Government Funds for Clinical Research (ALF)
- Swedish Research Council [2012-1883, 2017-02341]
- Finsenstiftelsen
- Swedish Research Council [2017-02341] Funding Source: Swedish Research Council
- Vinnova [2017-02341] Funding Source: Vinnova
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This study introduces an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. The results demonstrate compatibility with clinically relevant bacterial strains and confirm the effectiveness of antibacterial treatments, highlighting the formation of biofilms on the wound surface as well.
Wound infections are considered a major cause for wound-associated morbidity. There is a high demand for alternative, robust, and affordable methods that can provide relatable and reproducible results when testing topical treatments, both in research and in the pharmaceutical industry. Here we present an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. Utilizing this model, we demonstrate the potential of topical treatments after infecting the wounds with clinically significant bacteria, P. aeruginosa and S. aureus. We show that the method is compatible with several analytical tools used to analyze infection and antimicrobial effects. Both bacterial strains successfully infected the wound surface, as well as deeper regions of the tissue. Quantification of viable bacteria on the wound surface and in the tissue, longitudinal measurements of bioluminescence, fluorescence microscopy, and scanning electron microscopy were used to confirm the effects of antibacterial treatments. Furthermore, we show that biofilms are formed on the wound surface, indicating that the demonstrated method mirrors typical in vivo infections.
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