Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/ijms22094505
Keywords
Pelteobagrus fulvidraco; steroidogenesis-related genes; molecular characterization; promoter; transcriptional regulation
Funding
- National Natural Science Foundation of China [31572605]
- National Key R&D Program of China [2018YFD0900400]
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The study characterized the cDNA and promoter sequences of TSPO and SMAD4 genes in yellow catfish, explored their mRNA tissue expression, and deciphered their promoter regions. It also identified several transcription factors that influence the promoter activity of TSPO and SMAD4.
The steroid hormones are required for gonadal development in fish. The present study was undertaken to characterize the cDNA and promoter sequences of TSPO and SMAD4 genes in yellow catfish Pelteobagrus fulvidraco, explored the mRNA tissue expression and deciphered their promoter regions. Yellow catfish TSPO and SMAD4 shared the similar domains to the corresponding genes from other vertebrates. The TSPO and SMAD4 mRNAs were widely expressed in the detected tissues, but at different levels. Several transcription factors were predicted, such as Sp, GATA, AP1, SOX1, SRY, STAT, HNF4 alpha, PPAR gamma, Pu.1 and FOXL2. PPAR gamma overexpression increased but STAT3 overexpression reduced TSPO promoter activity, and FOXL2 overexpression inhibited the promoter activity of TSPO and SMAD4. The site mutation and EMSA analysis indicated that TSPO promoter possessed STAT3 and FOXL2 sites. Overall, our provided the novel understanding into the transcriptionally regulatory mechanisms of TSPO and SMAD4 in fish.
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